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        各類型色譜柱|菲羅門色譜柱|手性柱|氣相柱|SPE|高效液相色譜柱

        Solid-Core Mixed-Mode Stationary Phases

         

        SIELC Technologies has developed mixed-mode Columns based on solid-core-porous-shell particles. This separation media combines the efficiency of solid-core technology with the unique selectivity of Ion-Exchange (IE), reverse phase (RP), surface chemistry and ion-exchange HILIC surface.

        Combination of solid core particles and IE with RP or HILIC chemistry offers:

        • Fast separation
        • Working pressure within limits of conventional HPLC
        • Unique selectivity profile
        • Retention of compounds difficult to retain by RP chromatography
        • Increase column loading capacity
        • Adjustment of selectivity by mobile phase pH, ion strength, and organic concentration and composition

         

        Columns available in Solid-Core format with 2.7 μm particles:

        Column Ion-exchange Separation mode
        Primesep 100 Cation exchange RP
        Primesep SB Anion exchange RP
        Primesep S Cation exchange HILIC

         

        One disadvantage of the solid core support in HPLC is lower capacity when you compare it to regular through porous material. However, the mixed-mode materials demonstrated much higher loading capacity compared to regular RP phases (Nicola H. Davies, Melvin R. Euerby and David V. McCalley, Journal of Chromatography A, Volume 1138, Issues 1-2, 5 January 2007,  65-72). Thus, the mixed-mode surface chemistry compensates for loss of loading capacity of partially non-porous particles.

        Mixed mode columns demonstrate 2D characteristics with independent adjustment of degree of retention based on ionic and hydrophobic mechanism
        Reverse phase mechanism controls by amount of organic modifier in the mobile phase
        Ion-exchange mechanism controls by ion strength and pH of the mobile phase
        This property allows to resolve complex mixtures with polar, hydrophobic, ionic, and neutral compounds

         

        Some separation examples:

         

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